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Table 1 Sucrose phosphorylase expression data from literature and from this study

From: Whole cell-based catalyst for enzymatic production of the osmolyte 2-O-α-glucosylglycerol

Enzyme

Native host

Expression strain

Specific activity of cell-free extract

% of total soluble protein

References

BaSP

B. a. DSM 20083

B. adolescentis

0.84 U/mg

0.80%

van den Broek et al. [36]

B. a. DSM 20083

E. coli

19.356 U/L cell culture

van den Broek et al. [36]

B. a. LMG 10502

E. coli Rosetta 2

1.5 U/mL cell culture (OD 1)

(~ 6%)

Aerts et al. [37]

B. a. DSM 20083

E. coli BL21(DE3)

38 U/mg

33%

This study

LmSP

L. m. DSM 20193

L. m. DSM 20193

7 U/mg

3.7%

Koga et al. [38]

E. coli DH10B

48 U/mg

25%

Goedl et al. [28]

E. coli BL21(DE3)

55 U/mg

30%

This study

L. m. ATCC 12291

E. coli 1101 (slp-spl-1)

55.7 U/mg

30%

Kitoa et al. [39]

L. m. No. 165

E. coli JM109

4.6 U/mg

50%

Kawasaki et al. [40]

L.m

E. coli BL21(DE3)

~ 22 U/mg CDM

(~ 44 U/mg CFE)

25%

Su et al. [41]

L. m. NRRL B-1149

E. coli BL21(DE3)pLysS

1.49 U/mg

Specific activities of enzymes

Lee et al. [43]

L. m. NRRL B-742

E. coli BL21(DE3)pLysS

1.8 U/mg

Lee et al. [42]

TtSP

Thermoanaerobacterium thermosaccharolyticum

E. coli BL21(DE3)

25.7 U/mg

50%

Yao et al. [85]

BlSP

Bifidobacterium longum

E. coli BL21

13 U/mg

11%

Hui Zhang [86]

E. coli JM109

12.5 U/mg

10%

Shin et al. [87]