Fig. 8From: Whole cell-based catalyst for enzymatic production of the osmolyte 2-O-α-glucosylglycerolEvaluation of different catalyst preparations of E. coli LmSucP_pQE30 in GG synthesis. a Non-permeabilized cells, b cell lysate, c freeze-thawed cells, and d freeze-dried cells. Reactions were performed using 300 mM sucrose, 1.8 M glycerol, 40 U/mL LmSP phosphorolysis activity, 50 mM MES buffer at pH 7, 30 °C, 300 rpm for 24 hBack to article page