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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Whole cell-based catalyst for enzymatic production of the osmolyte 2-O-α-glucosylglycerol

Fig. 4

Bioconversions of a LmSucP_pQE30 and b BaSucP_pQE30 cell-free extracts. Reaction conditions: 300 mM sucrose, 1800 mM glycerol, 40 U/mL sucrose phosphorylase (phosphorolysis direction of the respective cell-free extract), 50 mM MES buffer at pH 7, 30 °C, 300 rpm for 24 h. c Product composition after 24 h GG production and d corresponding chromatograms of HPLC measurements

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