Fig. 3
From: Microbial production of ectoine and hydroxyectoine as high-value chemicals
Metabolic engineering modification strategy for ectoine and PHB co-production by H. bluephagenesis. a The reconstructed ectoine and PHB co-biosynthetic pathway in H. bluephagenesis. Modified metabolic fluxes of high efficiency involved in ectoine synthesis are shown in black bold lines. Genes doeA and ectD encoding ectoine hydrolase and ectoine hydroxylase, respectively, are deleted. b Application of two orthogonal inducible systems in ectoine synthesizes related genes. Two orthogonal inducible systems and fine-tuning of asd, lysC and ectABC genes