Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris

Table 1 Comparison of the main production parameters obtained in chemostat and fed-batch cultures with the producer clones at different specific growth rates (µ)

Clone	Operational mode	Nominal µ	Experimental µ	q_p	Y_P/X*
Clone	Operational mode	h⁻¹	h⁻¹	AU g_x⁻¹ h⁻¹	AU g_x⁻¹
GAP-C	Chemostat	0.050	0.046	1.16	24.9
		0.100	0.103	2.24	21.9
		0.150	0.149	2.74	18.4
PDF-C	Chemostat	0.050	0.047	7.20	153
		0.100	0.100	10.8	108
		0.150	0.155	10.8	70.1
	Fed-batch	0.050	0.042	9.20	219
	Fed-batch	0.100	0.087	13.1	150
UPP-C	Chemostat	0.050	0.052	10.3	197
		0.100	0.106	12.9	122
		0.150	0.156	11.4	72.7
	Fed-batch	0.050	0.051	11.1	217
	Fed-batch	0.100	0.084	9.95	102