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Table 1 Comparison of the main production parameters obtained in chemostat and fed-batch cultures with the producer clones at different specific growth rates (µ)

From: Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris

Clone Operational mode Nominal µ Experimental µ qp YP/X*
h−1 h−1 AU gx−1 h−1 AU gx−1
GAP-C Chemostat 0.050 0.046 1.16 24.9
0.100 0.103 2.24 21.9
0.150 0.149 2.74 18.4
PDF-C Chemostat 0.050 0.047 7.20 153
0.100 0.100 10.8 108
0.150 0.155 10.8 70.1
Fed-batch 0.050 0.042 9.20 219
0.100 0.087 13.1 150
UPP-C Chemostat 0.050 0.052 10.3 197
0.100 0.106 12.9 122
0.150 0.156 11.4 72.7
Fed-batch 0.050 0.051 11.1 217
0.100 0.084 9.95 102