Fig. 3
a CalB production kinetics (qP vs D) and CALB relative transcription levels determined in chemostat cultivations for the three expression systems studied. Transcript levels were normalized to the levels of the MTH1 transcript, which was used as housekeeping gene for the analysis. Error bars represent the standard deviation of two biological replicates. b Percentage of CALB relative transcription levels respect to the maximum observed for the corresponding expression system