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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Expression of a recombinant FLT3 ligand and its emtansine conjugate as a therapeutic candidate against acute myeloid leukemia cells with FLT3 expression

Fig. 3

SDS-PAGE analysis of the FL and DM1 conjugation products. a Nonreducing SDS-PAGE result of FL-DM1. Lane 1: FL-DM1; Lane 2: rhFL. b Western blot analysis of FL-DM1 under nonreducing SDS-PAGE. Lane 1: FL-DM1; Lane 2: rhFL. Primary antibody: anti-His tag antibody. c Reducing SDS-PAGE result of FL-DM1. Lane 1: FL-DM1; Lane 2: rhFL. d Reducing SDS-PAGE result of FL-SMCC-DM1. Lane 1: FL-SMCC-DM1; Lane 2: rhFL. E. Molecule weight result of FL-DM1 conjugation product analyzed by LC–MS

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