Skip to main content
Fig. 1 | Microbial Cell Factories

Fig. 1

From: Expression of a recombinant FLT3 ligand and its emtansine conjugate as a therapeutic candidate against acute myeloid leukemia cells with FLT3 expression

Fig. 1

Analysis of the expression and purification of rhFL. a SDS-PAGE analysis of the expression and purification of the rhFL protein. Lane 1: periplasmic fraction of induced BL21 (DE3) harboring the pET20-FL plasmid; Lane 2: Ni–NTA Sepharose column elution fraction; Lane 3: phenyl Sepharose column elution fraction. b Western blot analysis of rhFL protein. An anti-FL polyclonal antibody was used as the primary antibody. Lane 1: periplasmic fraction of induced BL21 (DE3) harboring the pET20-FL plasmid; lane 2: purified rhFL protein; lane 3: commercial recombinant FL as the positive control

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us