Fig. 5From: Pyridoxal-5′-phosphate-dependent enzyme GenB3 Catalyzes C-3′,4′-dideoxygenation in gentamicin biosynthesisDiscovery of 11 in GenB3-catalyzed reactions in vivo and in vitro. a HPLC-ELSD analysis of GenB3-catalyzed reactions in vitro with compound 2 at (i) 30 °C and (ii) 20 °C. b HPLC-ELSD analysis of genB4-disruption strains identified compound 11. c MS analysis of compound 11Back to article page