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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Pyridoxal-5′-phosphate-dependent enzyme GenB3 Catalyzes C-3′,4′-dideoxygenation in gentamicin biosynthesis

Fig. 4

GenB3-catalyzed dideoxygenation. a HPLC-ELSD analysis of mutant- strain fermentation products, showing the (i) wild-type, (ii) genB3-disruption strain (ΔgenB3), and (iii) ΔgenB3::genB3 (complementation of the ΔgenB3 mutant). b Ultraviolet–visible absorption spectrum of GenB3-catalyzed reaction at different reaction times, where UV absorption was measured every 2 min from 300 to 500 nm. c HPLC-ELSD analysis of (i) the compound 2 standard with L-Glu, (ii) the GenB3-catalyzed reaction without L-Glu, (iii) the GenB3-catalyzed reaction with L-Glu, (iv) with NaBH4 added after the reaction, (v) the compound 3 standard with L-Glu, and (vi) the GenB3-catalyzed reaction with compound 3. d HPLC-ELSD analysis. (i) The GenB3-catalyzed reaction with compound 1 was found to produce compound 9. (ii) L-Glu was added. (iii) An L-Glu-added sisomicin standard (Sis) was used. eh MS analysis of compounds 6, 7, 8, and 10

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