Fig. 4
From: Cell-based high-throughput screening of polysaccharide biosynthesis hosts
Cell-based high-throughput screening based on the coupling of cell growth with in vivo polysaccharide biosynthesis. An in vivo active biosensor responds to the intracellular concentration of a target metabolite that is associated with the cell’s ability to synthesize polysaccharides. One selects a suitable actuator (e.g., antibiotic, auxotroph, or toxin) that can directly select for and enrich high-yield strains. In selection conditions, the polysaccharide biosynthesis level is coupled with cell growth, so one can isolate cells with high productivity that also synthesize polysaccharides at a high level