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Table 2 Yields and rates of the three-stage process for 2,3-BDO production with the ATPase strain and the control strain (see also Fig. 4)

From: Increasing ATP turnover boosts productivity of 2,3-butanediol synthesis in Escherichia coli

  Stage 2: Aerobic phase (growth arrest by N limitation) Stage 3: Anaerobic phase (growth arrest by N limitation) Overall process (aerobic and anaerobic phase under growth arrest)
With acetoin consumption Acetoin depleted
Control strain ATPase strain Control strain ATPase strain Control strain ATPase strain Control strain ATPase strain
Time interval [h] 0–15.25 0–5.25 16.25–17.25 5.75–7.25 25.42–48 7.25–25.42 0–48 0–25.42
rGlucose [mmol/(gDW·h)] 2.30 ± 0.09 8.86 ± 0.19 1.33 ± 0.34 6.51 ± 0.93 0.94 ± 0.12 1.15 ± 0.25 1.35 ± 0.07 3.30 ± 0.17
r2,3-BDO [mmol/(gDW·h)] 0.18 ± 0.00 3.60 ± 0.08 3.54 ± 0.69 10.14 ± 1.08 1.25 ± 0.25 1.28 ± 0.22 0.83 ± 0.09 2.48 ± 0.17
rAcetoin [mmol/(gDW·h)] 0.35 ± 0.01 1.28 ± 0.09 − 3.29 ± 0.56 − 3.58 ± 0.34 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00
rPyruvate [mmol/(gDW·h)] 1.54 ± 0.04 6.93 ± 0.09 1.29 ± 0.38 − 0.63 ± 0.53 − 1.02 ± 0.10 − 0.95 ± 0.05 0.19 ± 0.02 0.99 ± 0.00
Y(2,3-BDO/Glucose) [mol/mol] 0.08 ± 0.00 0.41 ± 0.02 2.73 ± 0.49 1.57 ± 0.08 1.32 ± 0.10 1.13 ± 0.06 0.61 ± 0.04 0.75 ± 0.01
Y(Acetoin/Glucose) [mol/mol] 0.15 ± 0.01 0.14 ± 0.01 − 2.63 ± 0.71 − 0.56 ± 0.06 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00
Y(Pyruvate/Glucose) [mol/mol] 0.67 ± 0.02 0.78 ± 0.02 1.10 ± 0.60 − 0.11 ± 0.09 − 1.10 ± 0.03 − 0.86 ± 0.16 0.14 ± 0.01 0.30 ± 0.01
q2,3-BDO [mmol/(L·h)] 0.15 ± 0.00 2.23 ± 0.06 3.27 ± 0.64 6.18 ± 0.68 0.68 ± 0.15 0.70 ± 0.13 0.64 ± 0.07 1.44 ± 0.10
  1. Shown are the results for the (growth-decoupled) production stages 2 and 3. The switch from aerobic to anaerobic conditions was initiated when approximately 60% of the glucose had been consumed (see text). Negative rates and yields for acetoin and pyruvate indicate uptake of the respective compound. The values were calculated from triplicate experiments and the error ranges represent standard deviations.