Table 2 Yields and rates of the three-stage process for 2,3-BDO production with the ATPase strain and the control strain (see also Fig. 4)
From: Increasing ATP turnover boosts productivity of 2,3-butanediol synthesis in Escherichia coli
Stage 2: Aerobic phase (growth arrest by N limitation) | Stage 3: Anaerobic phase (growth arrest by N limitation) | Overall process (aerobic and anaerobic phase under growth arrest) | ||||||
|---|---|---|---|---|---|---|---|---|
With acetoin consumption | Acetoin depleted | |||||||
Control strain | ATPase strain | Control strain | ATPase strain | Control strain | ATPase strain | Control strain | ATPase strain | |
Time interval [h] | 0–15.25 | 0–5.25 | 16.25–17.25 | 5.75–7.25 | 25.42–48 | 7.25–25.42 | 0–48 | 0–25.42 |
rGlucose [mmol/(gDW·h)] | 2.30 ± 0.09 | 8.86 ± 0.19 | 1.33 ± 0.34 | 6.51 ± 0.93 | 0.94 ± 0.12 | 1.15 ± 0.25 | 1.35 ± 0.07 | 3.30 ± 0.17 |
r2,3-BDO [mmol/(gDW·h)] | 0.18 ± 0.00 | 3.60 ± 0.08 | 3.54 ± 0.69 | 10.14 ± 1.08 | 1.25 ± 0.25 | 1.28 ± 0.22 | 0.83 ± 0.09 | 2.48 ± 0.17 |
rAcetoin [mmol/(gDW·h)] | 0.35 ± 0.01 | 1.28 ± 0.09 | − 3.29 ± 0.56 | − 3.58 ± 0.34 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
rPyruvate [mmol/(gDW·h)] | 1.54 ± 0.04 | 6.93 ± 0.09 | 1.29 ± 0.38 | − 0.63 ± 0.53 | − 1.02 ± 0.10 | − 0.95 ± 0.05 | 0.19 ± 0.02 | 0.99 ± 0.00 |
Y(2,3-BDO/Glucose) [mol/mol] | 0.08 ± 0.00 | 0.41 ± 0.02 | 2.73 ± 0.49 | 1.57 ± 0.08 | 1.32 ± 0.10 | 1.13 ± 0.06 | 0.61 ± 0.04 | 0.75 ± 0.01 |
Y(Acetoin/Glucose) [mol/mol] | 0.15 ± 0.01 | 0.14 ± 0.01 | − 2.63 ± 0.71 | − 0.56 ± 0.06 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
Y(Pyruvate/Glucose) [mol/mol] | 0.67 ± 0.02 | 0.78 ± 0.02 | 1.10 ± 0.60 | − 0.11 ± 0.09 | − 1.10 ± 0.03 | − 0.86 ± 0.16 | 0.14 ± 0.01 | 0.30 ± 0.01 |
q2,3-BDO [mmol/(L·h)] | 0.15 ± 0.00 | 2.23 ± 0.06 | 3.27 ± 0.64 | 6.18 ± 0.68 | 0.68 ± 0.15 | 0.70 ± 0.13 | 0.64 ± 0.07 | 1.44 ± 0.10 |