Table 1 Growth rate, glucose uptake rate, specific production rates, product yields and volumetric productivities of the ATPase strain and the control strain in the different cultivation conditions investigated (cf. Figs. 2 and 3 and Additional file 1)
From: Increasing ATP turnover boosts productivity of 2,3-butanediol synthesis in Escherichia coli
Exponential growth (aerobic) | Growth arrest by N–limitation (aerobic) | Growth–arrest by N–limitation (microaerobic) | ||||
|---|---|---|---|---|---|---|
Control strain | ATPase strain | Control strain | ATPase strain | ATPase strain | ATPase strain | |
Time interval for rate and yield calculation [h] | 0–7 | 0–7 | 0–79 | 0–7 | 0–46.5 | 0–46.5 |
µ [h− 1] | 0.42 ± 0.01 | 0.20 ± 0.0 | 0* | 0 | 0 | 0 |
rGlucose [mmol/(gDW·h)] | 7.52 ± 0.88 | 11.24 ± 0.83 | 1.28 ± 0.09 | 9.26 ± 0.51 | 1.36 ± 0.38 | 2.20 ± 0.54 |
r2,3 − BDO [mmol/(gDW·h)] | 1.52 ± 0.11 | 3.18 ± 0.31 | 0.32 ± 0.02 | 3.58 ± 1.30 | 0.96 ± 0.31 | 1.90 ± 0.46 |
rAcetoin [mmol/(gDW·h)] | 1.55 ± 0.03 | 2.25 ± 0.17 | 0.38 ± 0.03 | 1.55 ± 0.27 | 0.00 ± 0.00 | 0.13 ± 0.14 |
rPyruvate [mmol/(gDW·h)] | 3.34 ± 0.18 | 10.38 ± 0.28 | 1.01 ± 0.03 | 10.22 ± 0.62 | 0.00 ± 0.00 | 0.02 ± 0.02 |
Y(2,3 − BDO/Glucose) [mol/mol] | 0.20 ± 0.02 | 0.29 ± 0.05 | 0.25 ± 0.03 | 0.39 ± 0.15 | 0.70 ± 0.05 | 0.87 ± 0.05 |
Y(Acetoin/Glucose) [mol/mol] | 0.21 ± 0.03 | 0.20 ± 0.02 | 0.30 ± 0.01 | 0.17 ± 0.03 | 0.00 ± 0.00 | 0.05 ± 0.06 |
Y(Pyruvate/Glucose) [mol/mol] | 0.46 ± 0.05 | 0.93 ± 0.07 | 0.79 ± 0.05 | 1.11 ± 0.10 | 0.00 ± 0.00 | 0.01 ± 0.01 |
q2,3 − BDO [mmol/(L·h)] | 0.73 ± 0.07 | 0.47 ± 0.05 | 0.18 ± 0.03 | 0.94 ± 0.17 | 0.45 ± 0.14 | 0.86 ± 0.20 |