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Table 1 Growth rate, glucose uptake rate, specific production rates, product yields and volumetric productivities of the ATPase strain and the control strain in the different cultivation conditions investigated (cf. Figs. 2 and 3 and Additional file 1)

From: Increasing ATP turnover boosts productivity of 2,3-butanediol synthesis in Escherichia coli

  Exponential growth (aerobic) Growth arrest by N–limitation (aerobic) Growth–arrest by N–limitation (microaerobic)
  Control strain ATPase strain Control strain ATPase strain ATPase strain ATPase strain
Time interval for rate and yield calculation [h] 0–7 0–7 0–79 0–7 0–46.5 0–46.5
µ [h− 1] 0.42 ± 0.01 0.20 ± 0.0 0* 0 0 0
rGlucose [mmol/(gDW·h)] 7.52 ± 0.88 11.24 ± 0.83 1.28 ± 0.09 9.26 ± 0.51 1.36 ± 0.38 2.20 ± 0.54
r2,3 − BDO [mmol/(gDW·h)] 1.52 ± 0.11 3.18 ± 0.31 0.32 ± 0.02 3.58 ± 1.30 0.96 ± 0.31 1.90 ± 0.46
rAcetoin [mmol/(gDW·h)] 1.55 ± 0.03 2.25 ± 0.17 0.38 ± 0.03 1.55 ± 0.27 0.00 ± 0.00 0.13 ± 0.14
rPyruvate [mmol/(gDW·h)] 3.34 ± 0.18 10.38 ± 0.28 1.01 ± 0.03 10.22 ± 0.62 0.00 ± 0.00 0.02 ± 0.02
Y(2,3 − BDO/Glucose) [mol/mol] 0.20 ± 0.02 0.29 ± 0.05 0.25 ± 0.03 0.39 ± 0.15 0.70 ± 0.05 0.87 ± 0.05
Y(Acetoin/Glucose) [mol/mol] 0.21 ± 0.03 0.20 ± 0.02 0.30 ± 0.01 0.17 ± 0.03 0.00 ± 0.00 0.05 ± 0.06
Y(Pyruvate/Glucose) [mol/mol] 0.46 ± 0.05 0.93 ± 0.07 0.79 ± 0.05 1.11 ± 0.10 0.00 ± 0.00 0.01 ± 0.01
q2,3 − BDO [mmol/(L·h)] 0.73 ± 0.07 0.47 ± 0.05 0.18 ± 0.03 0.94 ± 0.17 0.45 ± 0.14 0.86 ± 0.20
  1. *Minor growth at the beginning is not considered
  2. The values were calculated from triplicate experiments and the error ranges represent standard deviations. The time intervals for rate and yield calculations were chosen such that they covered the exponential growth phase (growth-coupled production) or the interval where glucose was not yet depleted (under growth arrest)