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Table 4 Transgalactosylation products identified by ESI–MS

From: Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase

Acceptor:Donor

Transgalactosylation products

MNa+ Theoretical (Da)

MNa+ observed (Da)

(ONP-β-Gal: ONP-β-Gal)

1. (ONP-β-Gal + Gal) Na+

486.408

486.142

2. (ONP-β-Gal + 2 Gal) Na+

648.565

648.205

3. (ONP-β-Gal + 3Gal) Na+

810.722

810.277

(ONP-β-Gal/PNP-β-Glu: ONP-β-Gal)

1. (ONP-β-Gal/PNP-β-Glu + Gal/Glu) Na+

486.408

486.150

2. (ONP-β-Gal/PNP-β-Glu + 2Gal/2Glu) Na+

648.565

648.224

(PNP-β-Xyl:ONP-β-Gal)

1. (ONP-β-Gal + Gal) Na+

486.408

486.159

2. (ONP-β-Gal + 2Gal) Na+

648.565

648.195

3. (PNP-β-Xyl + Gal) Na+

456.382

456.146

4. (PNP-β-Xyl + Gal + Gal) Na+

618.539

618.211

(D-Lactose:D-Lactose)

1. (Lactose + Gal) Na+

527.477

527.202

  1. All components were detected as adducts with Na+. The observed and theoretical molecular weights are reported. The ESI–MS analysis cannot distinguish between the epimer Gal and Glu, which are reported as alternatives in the interpretation of MS spectra obtained with the couple ONP-β-Gal/PNP-β-Glu: ONP-β-Gal as acceptor and donor, respectively