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Table 2 Substrate specificity of BcGalB

From: Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase

Substrate

Specific activity (U/mg)

ONP-β-gal

4373.4 \(\pm\) 77.6

PNP-β-gal

795.9 \(\pm\) 3.9

PNP-α-ara

328.5 \(\pm\) 11.6

PNP-β-xyl

N.D

PNP-β-glu

N.D

D-lactose

1283.0 \(\pm\) 24.7

  1. The highest activity of BcGalB is toward ONP-β-gal, whilst the enzyme is not active on the arylic compounds PNP-β-xyl and PNP-β-glu. These latter two substrates are shown since they have been used in transglycosylation reactions (see below). N.D. not detected
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Microbial Cell Factories

ISSN: 1475-2859

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