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Table 1 Predicted GH in the genome of B. coagulans MA-13

From: Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase

NCBI Reference Sequence

GH family

Signal Peptide

RAST

annotation

WP_195850490.1

GH3

N

β-glycosyl hydrolase

WP_019720988.1

CBM34 + GH13_20

N

Neopullulanase (EC 3.2.1.135)

WP_133536160.1

GH13_31

N

Oligo-1,6-glucosidase (EC 3.2.1.10)

WP_195850265.1

GH13_31

N

Oligo-1,6-glucosidase (EC 3.2.1.10)

WP_133536961.1

GH13_5

N

Glucan 1,4-α-maltohexaosidase (EC 3.2.1.98)

WP_195850265.1

CBM48 + GH13_9

N

1,4-α-glucan (glycogen) branching enzyme (EC 2.4.1.18)

WP_061575462.1

GH0

N

Phosphorylase b kinase regulatory subunit β

WP_133537568.1

CBM50 + GH18

N

Spore cortex-lytic enzyme, N-acetylglucosaminidase SleL

WP_133536804.1

CBM50 + GH18

N

spore peptidoglycan hydrolase (N-acetylglucosaminidase) (EC 3.2.1.-)

WP_133537667.1

GH32

N

Sucrose-6-phosphate hydrolase (EC 3.2.1.26)

WP_133537615.1

GH36

N

α-galactosidase (EC 3.2.1.22)

WP_133536219.1

GH42

N

β-galactosidase (EC 3.2.1.23)

WP_133536548.1

GH65

N

α,α-trehalose phosphorylase (2.4.1.64)

WP_133536158.1

GH65

N

Maltose phosphorylase (EC 2.4.1.8)

WP_133536578.1

GH65

N

Maltose phosphorylase (EC 2.4.1.8)

WP_133537168.1

GH70

Y (1–34)

hypothetical protein

WP_195850162.1

GH73

N

endo-β-N-acetylglucosaminidase (EC 3.2.1.96)

  1. The automated CAZyme annotation has been carried out using dbCAN2 metaserver, integrated with HMMER, DIAMOND and Hotprep databases [35]. The presence of signal peptides in the proteins are reported as: Y = 100%, N = 0%. GH Glycosyl hydrolases, CBM carbohydrate-binding modules