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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Prebiotic properties of Bacillus coagulans MA-13: production of galactoside hydrolyzing enzymes and characterization of the transglycosylation properties of a GH42 β-galactosidase

Fig. 6

Time course of transgalactosylation reaction detected by TLC analysis. Homo-condensation reactions using ONP-β-gal as donor and acceptor (a). Hetero-condensation reactions performed with PNP-β-glu (b) and PNP-β-xyl (c) as acceptors and ONP-β-gal as donor. Transgalactosylation reactions using D-lactose as substrate (d). Red, blue and green circles show the UV signals obtained from the arylic group of ONP-β-gal, PNP-β-glu, and PNP-β-xyl respectively. STD1: Standard with ONP-β-gal and D-galactose, STD2: Standard with PNP-β-glu and D-glucose, STD3: Standard with PNP-β-xyl and D-xylose, STD4: Standard with D-glucose, STD5: Standard with D-galactose and D-lactose, S: Samples collected at different times (min), B: Blank at different times, SD: Sample diluted, BD: Blank diluted, TP: Transgalactosylation products

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