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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Comparative metabolomics analysis of amphotericin B high-yield mechanism for metabolic engineering

Fig. 7

AmB production associated with genes overexpression and the fermentation time courses. a AmB production associated with genes overexpression in various engineered strains, the genetically engineered strains were all constructed from primitive strain, mutant S. nodosus. Control represented mutant S. nodosus strain, pJTU1278 represented mutant S. nodosus with plasmid pJTU1278. PurN, metH, glyA, metF, purH and fmt represent overexpression of gene purN, metH, glyA, metF, purH and fmt. Samples were collected from soluble fermentation at 120 h, and the AmB concentration and ration of AmA were detected and analyzed, respectively. Symbol ‘*’ indicates the experimental strain compare with the mutant strain. More p-value details about symbols are * indicates p < 0.05, ** indicates p < 0.01. b Fermentation time course for mutant S. nodosus, pJTU1278, GlyA and MetH. Mutant S.nodosus and pJTU1278 were primitive strain and the strain with empty vector, respectively. MetH and glyA represented overexpression of gene metH and glyA. Error bars showed standard derivation among three experiments

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