Skip to main content
Fig. 5 | Microbial Cell Factories

Fig. 5

From: Comparative metabolomics analysis of amphotericin B high-yield mechanism for metabolic engineering

Fig. 5

The relative abundance of various intracellular metabolites in different fermentation period. Metabolites were analyzed to investigate the differences during the whole fermentation process of S. nodosus, including metabolism of central carbon, fatty acids, triglycerides, amino acids, purine, folate biosynthesis and one carbon pool by folate. a Central carbon metabolism. Arb arbutin, Glu glucose, Lac lactate, S7P sedoheptulose-7-phosphate, Sor sorbitol, T6P tagatose-6-phosphate, Raf raffinose, Sal salicin, Suc succinic acid. b Fatty acid biosynthesis. 1 lysophosphatidylcholine, PA phosphatidic acid, PC phosphatidyl choline, PE phosphatidyl ethanolamine, C13 tridecanoic acid, C14 myristic acid, C15 pentadecanoic acid, C16 palmitic acid, C17 heptadecanoic acid, C18 stearic acid, iC14 isomyristic acid. c Triglycerides metabolism. 2, TG(10:0/10:0/8:0); 3, TG(10:0/8:0/8:0); 4, TG(10:0/i-20:0/14:0); 5, TG(12:0/12:0/12:0); 6, TG(14:0/18:0/16:0); 7, TG(16:0/15:0/18:0); 8, TG(19:0/22:0/10:0); 9, TG(20:0/i-12:0/i-18:0); 10, TG(8:0/12:0/10:0); 11, TG(8:0/8:0/8:0); 12, TG(8:0/i-24:0/i-15:0); 13, TG(i-20:0/i-20:0/8:0); 14, TG(i-24:0/8:0/14:0); 15, TG(i-24:0/i-13:0/10:0). d Amino acid metabolism. MeHA methylhistamine, EGT ergothioneine, Hal histidinal, Arg arginine, Cys cysteine, Gln Glutamine, GSH glutathione, Hol histidinol, Met methionine, Ser serine, 16 Phosphoribosyl-AMP, SAM S-Adenosylmethionine, THDP tetrahydrodipicolinate. e Purine metabolism. A adenine, AICA 5-aminoimidazole-4-carboxamide, AIR 5-aminoimidazole ribonucleotide, dIMP deoxyinosine monophosphate, FGAM formyl glycinamidine ribonucleotide, FGAR formyl-glycinamide ribonucleotide, I inosine. f Folate biosynthesis and one carbon pool by folate. 17, 5,10-methylenetetrahydrofolic acid; 18, 5-formiminotetrahydrofolic acid; 19, 5-methyltetrahydrofolic acid; THF, tetrahydrofolic acid. The X-axis showed the relative abundance of metabolites. The Y-axis showed the different metabolites. Each value shown represented the mean of five independent experiments and the error bars represented standard deviations of five values

Back to article page