Table 1 Strains and plasmids used in this study
From: Efficient bioconversion of raspberry ketone in Escherichia coli using fatty acids feedstocks
Strain | Genotype | Source |
|---|---|---|
E. coli BW25113/F׳ | rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 [F׳ proAB lacIqZΔM15 Tn10 (Tetr)] | CGSC |
FA09 | E. coli BW25113/F׳, ΔfadR, PCPA1-fadD, P119-fadL, ΔsthA, PCPA1-pntAB | [28] |
CC1 | FA09, ΔpoxB:: Pfrd3-AT4CL1 | This study |
CC2 | CC1, ΔfabB:: P119-BT-CA | This study |
CC3 | CC2, Pfrd3-pntAB | This study |
CR1 | E. coli BW25113/F׳ carrying pLB1a-RB and pYB1s- 4CL | This study |
CR2 | E. coli BW25113/F carrying pYB1s-RB and pLB1a-4CL | This study |
CR3 | E. coli BW25113/F׳ carrying pLB1a-RB4 | This study |
CR4 | E. coli BW25113/F׳ carrying pYB1s-RB4 | This study |
CR5 | FA09 carrying pYB1s-RB4 | This study |
CR6 | CC1 carrying pYB1s-RB4 | This study |
CR7 | CC2 carrying pYB1s-RB4 | This study |
CR8 | CC3 carrying pYB1s-RB4 | This study |
Plasmid | Description | Source |
|---|---|---|
pLB1a | araBAD promoter, R6k ori, Ampr | Our laboratory |
pYB1s | araBAD promoter, p15A ori, Strr | Our laboratory |
pKD46 | Temperature-sensitive vector carrying Red recombinase, Ampr | [42] |
pSB1s-Cre | Temperature-sensitive vector carrying Cre recombinase, Strr | Our laboratory |
pCP20 | Temperature-sensitive vector carrying FLP recombinase, Ampr | [42] |
pLB1a-RB | pLB1a containing Rubus idaeus RZS1 gene and Rheum palmatum BAS gene | This study |
pLB1a-4CL | pLB1a containing Arabidopsis thaliana 4CL1 gene | This study |
pYB1s-RB | pYB1s containing Rubus idaeus RZS1 gene and Rheum palmatum BAS gene | This study |
pYB1s- 4CL | pYB1s containing Arabidopsis thaliana 4CL1 gene | This study |
pLB1a-RB4 | pLB1a containing Rubus idaeus RZS1 gene, Rheum palmatum BAS gene and Arabidopsis thaliana 4CL1 gene | This study |
pYB1s-RB4 | pYB1s containing Rubus idaeus RZS1 gene, Rheum palmatum BAS gene and Arabidopsis thaliana 4CL1 gene | This study |
pYfrd11s-GFP | frd1 promoter, p15A ori, Strr, GFP gene | This study |
pYfrd21s-GFP | frd2 promoter, p15A ori, Strr, GFP gene | This study |
pYfrd31s-GFP | frd3 promoter, p15A ori, Strr, GFP gene | This study |
pYfrd41s-GFP | frd4 promoter, p15A ori, Strr, GFP gene | This study |