Fig. 4

RK production under different fermentation conditions for CR5. MCM medium containing 5 mM p-coumaric acid was used for F1-F7 fermentation. The concentrations of different compositions used were: F1: none; F2: 0.2% FA; F3: 0.5% FA; F4:1% FA; F5: 1%FA + 0.5%glucose; F6: 1%FA + 0.5% glycerol; F7: 1%FA + 1% glycerol. Fermentations were performed at 30 °C and 200 rpm for 24 h. MCM7 medium was used for C1 and C2 bioconversion. C1: cells were induced by MCM7 medium and harvested, then suspended in MCM7 medium containing 5 mM p-coumaric acid (OD₆₀₀ = 30); C2: Cells from CR5 cultured in MCM7 medium were harvested and suspended in MCM7 medium (OD₆₀₀ = 30). Bioconversions were performed at 30 °C and 200 rpm for 24 h