Fig. 2
From: Efficient bioconversion of raspberry ketone in Escherichia coli using fatty acids feedstocks
Strains optimization and RK production using a different strategy. a Construction of different RK strains. Thick lines indicate medium-copy-number plasmids; thin lines indicate low-copy-number plasmids; yellow arrows represent RiRZS1; blue arrows represent RpBAS, and green arrows represent At4CL1. b RK production by five different strains containing different plasmids singly or in combination. One-step fermentations were performed at 30 °C and 200 rpm for 24 h. CM medium containing 5 mM p-coumaric acid was used for RK production. c RK production by one-step fermentation by strain CR4. CM medium and M9-glucose medium containing 5 mM p-coumaric acid was used for RK production. One-step fermentations were performed at 30 °C and 200 rpm for 48 h. d RK production by bioconversion by strain CR4. CR4 was induced in the shake flasks containing CM medium at 30 °C and 200 rpm for 16 h. Cells were harvested, then suspended in CM medium or M9-glucose medium containing 5 mM p-coumaric acid (OD600 = 30). Bioconversions were performed at 30 °C and 200 rpm for 48 h