Table 3 The experimental design and response of lytic Staphylococcus aureus phage pSa-3 production
Standard Order | Glycerol X1 | Glycine X2 | CaCl2 X3 | Observed values (\(\times\)1010Â PFU/mL) | Predicted values (\(\times\)1010Â PFU/mL) |
|---|---|---|---|---|---|
1 | − 1 | − 1 | 1 | 1.74 ± 1.42 | 4.81 |
2 | 1 | − 1 | − 1 | 4.54 ± 0.17 | 3.78 |
3 | 1 | 1 | 1 | 3.57 ± 0.29 | 3.78 |
4 | − 1 | 1 | − 1 | 8.31 ± 0.93 | 4.81 |
5 | 0 | 0 | 0 | 5.11 ± 0.41 | 4.36 |
6 | 0 | 0 | 0 | 5.12 ± 0.23 | 4.36 |
7 | 1 | 1 | − 1 | 2.01 ± 0.21 | 3.77 |
8 | 0 | 0 | 0 | 5.09 ± 0.18 | 4.36 |
9 | − 1 | − 1 | − 1 | 9.41 ± 2.08 | 4.81 |
10 | 1 | − 1 | 1 | 2.88 ± 0.40 | 3.79 |
11 | − 1 | 1 | 1 | 4.05 ± 1.85 | 4.81 |
12 | 0 | 0 | 0 | 5.06 ± 0.28 | 4.36 |
13 | 1.633 | 0 | 0 | 1.98 ± 0.42 | 3.35 |
14 | 0 | 0 | − 1.633 | 5.26 ± 0.26 | 4.36 |
15 | 0 | 0 | 1.633 | 6.83 ± 0.69 | 4.36 |
16 | 0 | − 1.633 | 0 | 7.07 ± 1.10 | 4.36 |
17 | 0 | 1.633 | 0 | 7.60 ± 1.24 | 4.35 |
18 | 0 | 0 | 0 | 5.20 ± 0.26 | 4.36 |
19 | 0 | 0 | 0 | 5.13 ± 0.31 | 4.36 |
20 | − 1.633 | 0 | 0 | 4.05 ± 0.75 | 5.03 |