Table 2 The Central Composite Design (CCD) of experiments and response of Staphylococcus aureus phage pSa-3 production
Standard order | Variables (coded value) | Variables (experimental value) | ||||
|---|---|---|---|---|---|---|
Glycerol X1 | Glycine X2 | CaCl2 X3 | Glycerol (mg) | Glycine (mg) | CaCl2 (mM) | |
1 | − 1 | − 1 | 1 | 250.0 | 25.0 | 15.0 |
2 | 1 | − 1 | − 1 | 750.0 | 25.0 | 5.0 |
3 | 1 | 1 | 1 | 750.0 | 75.0 | 15.0 |
4 | − 1 | 1 | − 1 | 250.0 | 75.0 | 5.0 |
5 | 0 | 0 | 0 | 500.0 | 50.0 | 10.0 |
6 | 0 | 0 | 0 | 500.0 | 50.0 | 10.0 |
7 | 1 | 1 | − 1 | 750.0 | 75.0 | 5.0 |
8 | 0 | 0 | 0 | 500.0 | 50.0 | 10.0 |
9 | − 1 | − 1 | − 1 | 250.0 | 25.0 | 5.0 |
10 | 1 | − 1 | 1 | 750.0 | 25.0 | 15.0 |
11 | − 1 | 1 | 1 | 250.0 | 75.0 | 15.0 |
12 | 0 | 0 | 0 | 500.0 | 50.0 | 10.0 |
13 | 1.633 | 0 | 0 | 908.0 | 50.0 | 10.0 |
14 | 0 | 0 | − 1.633 | 500.0 | 50.0 | 1.8 |
15 | 0 | 0 | 1.633 | 500.0 | 50.0 | 18.1 |
16 | 0 | − 1.633 | 0 | 500.0 | 9.0 | 10.0 |
17 | 0 | 1.633 | 0 | 500.0 | 90.0 | 10.0 |
18 | 0 | 0 | 0 | 500.0 | 50.0 | 10.0 |
19 | 0 | 0 | 0 | 500.0 | 50.0 | 10.0 |
20 | − 1.633 | 0 | 0 | 91.0 | 50.0 | 10.0 |