Table 2 The primers and synthetic DNA sequences used in this study
From: Improving l-threonine production in Escherichia coli by elimination of transporters ProP and ProVWX
Names | Sequence (5′–3′) |
|---|---|
proPf1 | TGCCGCCTTGTATGAGTG |
proPr1 | CAAATCGGCAATCTCGTGACCGAGAAACCTTGTGCC |
proPf2 | GGCACAAGGTTTCTCGGTCACGAGATTGCCGATTTG |
proPr2 | CCCATTGCCATTCACAGC |
proP-sgRAN-F | GCATTACTCCGAAGACCACGGTTTTAGAGCTAGAAATAGC |
sgRNA-R | AGTATTATACCTAGGACTGAGCTAG |
Y-pTargetF-F | CGTATTACCGCCTTTGAGTGAG |
Y-pTargetF-R | GCTTATGGAGCTGCACATGAACT |
proVWXf1 | AGCAGTCCACGGTTACTACAT |
proVWXr1 | GCCCTCTTTGTAGCGACTTTCCGGCCAATTCCATACC |
proVWXf2 | GTATGGAATTGGCCGGAAAGTCGCTACAAAGAGGGC |
proVWXr2 | TCAAACATATCACCGAGGG |
proVWX-sgRAN-F | ACGACTGCACCGACTGACGGGTTTTAGAGCTAGAAATAGC |
betABITf1 | ATTGAACATTCGCAACAGC |
betABITr1 | CGGACATCTCGTCGTAACCCTACAGCAAAGAGCAGGTGAT |
betABITf2 | ATCACCTGCTCTTTGCTGTAGGGTTACGACGAGATGTCCG |
betABITr2 | TGGCGTTGTCTGGTTTATTT |
betABIT-sgRAN-F | ATCTACCCACCGATTAACCGGTTTTAGAGCTAGAAATAGC |
proCf1 | CCTTTGTTGGGTAATCCTCT |
proCr1 | CCATACACTTCGTCATCGCTGTCTTTATTCAGGCTGGAGG |
proCf2 | CCTCCAGCCTGAATAAAGACAGCGATGACGAAGTGTATGG |
proCr2 | CGGTAGCGATGTGATTGG |
proC-sgRAN-F | GATATGGTCTGCTCACCGGGGTTTTAGAGCTAGAAATAGCTAGC |
RT-16sRNA-F | TCGGGAACCGTGAGACAGG |
RT-16sRNA-R | CCGCTGGCAACAAAGGATAAG |
RT-glk-F | CTTGCTCTGTGTGATATTGC |
RT-glk-R | GTCATCGCCACCCAGTCAC |
RT-pgi-F | GTTCTGGGACTGGGTTGGCG |
RT-pgi-R | GCGTGGTGGAGAAATGCTTG |
RT-zwf-R | TAAACGTCTGCCGACCAAAT |
RT-zwf-R | AGATGCGTCTGATTAAAGGT |
RT-ptsG-F | TTTTGTTGTAATGCGGTGGTT |
RT-ptsG-R | GCAGGGAAAGTGTGTTGTCG |
RT-crr-F | AACCCTGAAGAACTGATTGGC |
RT-crr-R | GCACCGCAACTTCACTCTTC |
RT-galP-F | CACAACAAGTCCAGCA TTCC |
RT-galP-R | AGCCGCACCTTTACCATC |
RT-mglB-F | TGAAGAAA TCGCCTCTAAAC |
RT-mglB-R | GCAAAGAAGCCAAGA TACTC |
RT-ndh-F | TGGGACGCAAGAAAAAAGC |
RT-ndh-R | CACGATCAATATCAATGACGG |
RT-betA-F | GCGTCACTACCTCCAAACCC |
RT-betA-R | TCCATCGGACCAAAACCTTC |
RT-betB-F | ACTCGCAAAACTGGAAACCC |
RT-betB-R | GCAATCTGGATCGGGTAGTTC |
RT-betT-F | CACCTGCTCTTTGCTGTAGTCC |
RT-betT-R | CAGTTGGGTCATCTGGATTTGT |