A bifunctional enzyme belonging to cytochrome P450 family involved in the O-dealkylation and N-dealkoxymethylation toward chloroacetanilide herbicides in Rhodococcus sp. B2

Table 2 Analysis of variance (ANOVA) for the response surface quadratic model

Source	Sum of squares	Degrees of freedom	Mean square	F-value	p-value
Model	1489.53	9	165.5	21.17	< 0.0001	Significant
A-pH	54.42	1	54.42	6.96	0.0248
B-T	5.29	1	5.29	0.6762	0.4301
C-I	89.97	1	89.97	11.51	0.0069
AB	87.12	1	87.12	11.14	0.0075
AC	45.51	1	45.51	5.82	0.0365
BC	4.62	1	4.62	0.591	0.4598
A²	1118.5	1	1118.5	143.06	< 0.0001
B²	152.73	1	152.73	19.53	0.0013
C²	8.27	1	8.27	1.06	0.3279
Residual	78.19	10	7.82
Lack of fit	60.88	5	12.18	3.52	0.0969	Not significant
Pure error	17.31	5	3.46
Cor total	1567.72	19

ISSN: 1475-2859