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Fig. 4 | Microbial Cell Factories

Fig. 4

From: A bifunctional enzyme belonging to cytochrome P450 family involved in the O-dealkylation and N-dealkoxymethylation toward chloroacetanilide herbicides in Rhodococcus sp. B2

Fig. 4

Physical map of the putative transposable element containing EthRABCDB2 gene cluster in Rhodococcus sp. B2 and degradation pathway of chloroacetanilide herbicides. a Arrows indicate the sizes, locations, and directions of transcription of the ORFs. Complementation of the EthRABCDB2-disrupted mutants with different regions is illustrated below the physical map. b The difference of the amino acid sequences of EthB from strain B2 and T3-1. Highlighted characters represent the different residues. c Proposed degradation pathway of pretilachlor, alachlor, acetochlor, propisochlor and butachlor by EthABDB2. I, pretilachlor. II, Alachlor, R1 = CH3CH2-, R2 = CH3-; Acetochlor, R1 = CH3-, R2 = CH3CH2-; Butachlor, R1 = CH3CH2-, R2 = CH3CH2CH2CH2-: Propisochlor, R1 = CH3-,R2 = (CH3)2CH-.Strain B2 cannot further metabolize three metabolites

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Microbial Cell Factories

ISSN: 1475-2859

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