Strain or plasmid | Description | Antibiotic resistancea | Source or reference |
---|---|---|---|
Pantoea ananatis | |||
SC17(0) | λ Red resistant strain | – | [19] |
SWITCH-PphoC Δgcd | SC17(0) ΔampC::Ptac-KDyI ΔampH::PphoC-mvaES ΔcrtEXYIB-crtZ::Ptac-mvk Δgcd | – | [26] |
IP03 | SWITCH-PphoC Δgcd ΔL-ldh::PphoC-mvaES | – | This study |
IP04 | IP03 ΔadhE::Ptac-φ10-mvk | – | This study |
Plasmid | |||
pSol-AaLINS | pSol plasmid for expression of AaLINS under the control of a rhamnose-inducible promoter | Km | This study |
pSol-HisAaLINS | pSol plasmid for expression of hexahistidine-tagged AaLINS | Km | This study |
pSol-BLAAaLINS | pSol plasmid for expression of AaLINS fused with β-lactamase from Chromohalobacter sp. 560 joined to hexahistidine | Km | This study |
pACYC177-Ptac-AaLINS-ispA* (pAaLINS-ispA*) | pACYC177 derivative for expression of AaLINS and ispA* under the control of tac promoter | Km | [15] |
pBLAAaLINS-ispA* | pACYC177 derivative for expression of the gene of AaLINS fused with β-lactamase from Chromohalobacter sp. 560 joined to hexahistidine and ispA* under the control of tac promoter | Km | This study |