Skip to main content

Table 2 Bacterial strains and plasmids used in this study

From: Fermentative production of enantiopure (S)-linalool using a metabolically engineered Pantoea ananatis

Strain or plasmid

Description

Antibiotic resistancea

Source or reference

Pantoea ananatis

   

 SC17(0)

λ Red resistant strain

[19]

 SWITCH-PphoC Δgcd

SC17(0) ΔampC::Ptac-KDyI ΔampH::PphoC-mvaES ΔcrtEXYIB-crtZ::Ptac-mvk Δgcd

[26]

 IP03

SWITCH-PphoC Δgcd ΔL-ldh::PphoC-mvaES

This study

 IP04

IP03 ΔadhE::Ptac-φ10-mvk

This study

Plasmid

   

 pSol-AaLINS

pSol plasmid for expression of AaLINS under the control of a rhamnose-inducible promoter

Km

This study

 pSol-HisAaLINS

pSol plasmid for expression of hexahistidine-tagged AaLINS

Km

This study

 pSol-BLAAaLINS

pSol plasmid for expression of AaLINS fused with β-lactamase from Chromohalobacter sp. 560 joined to hexahistidine

Km

This study

 pACYC177-Ptac-AaLINS-ispA* (pAaLINS-ispA*)

pACYC177 derivative for expression of AaLINS and ispA* under the control of tac promoter

Km

[15]

 pBLAAaLINS-ispA*

pACYC177 derivative for expression of the gene of AaLINS fused with β-lactamase from Chromohalobacter sp. 560 joined to hexahistidine and ispA* under the control of tac promoter

Km

This study

  1. Km kanamycin