Fermentative production of enantiopure (S)-linalool using a metabolically engineered Pantoea ananatis

Table 1 (S)-Linalool production in test-tube cultivation

Host strain	Plasmid	(S)-linalool (g/L)^a	Consumed glucose (g/L)^a	Yield (%)^b
SWITCH-PphoC Δgcd	pACYC177	ND^c	43 ± 3.3	–
SWITCH-PphoC Δgcd	pAaLINS-ispA*	3.4 ± 0.2	60 ± 0.0	5.6 ± 0.3
SWITCH-PphoC Δgcd	pBLAAaLINS-ispA*	3.0 ± 0.0	60 ± 0.0	5.0 ± 0.0
IP03	pAaLINS-ispA*	0.8 ± 0.0	43 ± 0.6	1.9 ± 0.0
IP03	pBLAAaLINS-ispA*	4.0 ± 0.2	60 ± 0.0	6.6 ± 0.4
IP04	pACYC177	ND	38 ± 1.7	–
IP04	pAaLINS-ispA*	1.0 ± 0.0	36 ± 0.6	2.8 ± 0.1
IP04	pBLAAaLINS-ispA*	4.7 ± 0.3	60 ± 0.0	7.9 ± 0.2

All strains were cultivated for 48 h under biphasic fermentation using isopropyl myristate. Data are expressed as the mean ± SD of at least three biological replicates
^aGlucose and (S)-linalool concentrations are represented by dividing the total amounts by the volume of aqueous culture
^bYield was calculated as grams of product per grams of consumed glucose and is expressed as a percentage. Carbon sources contained in 2 g/L of Bacto yeast extract was not considered in this calculation
^cND, Not detected

ISSN: 1475-2859