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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Fermentative production of enantiopure (S)-linalool using a metabolically engineered Pantoea ananatis

Fig. 2

SDS–PAGE gels illustrating total and soluble expression levels of solubility-tag fused AaLINS variants. a, b Gels stained with Coomassie Brilliant Blue; c, d Gels stained with an anti-polyhistidine label (a fluorescent dye conjugated to nickel-nitrilotriacetic acid complex). CH, SF, and M denote crude homogenate, soluble fraction, and protein standard, respectively. Samples were prepared from SC17(0) harboring each pSol plasmid grown in LB medium containing rhamnose. Each applied sample contained 10 µg of soluble proteins. Arrow shows each AaLINS variant. AFV, AFV1–99 protein from Acidianus filamentous virus 1; BLA, halophilic β-lactamase from Chromohalobacter sp. 560; 6×His, hexahistidine; MBP, maltose binding protein; SlyD, FKBP-type peptidyl-prolyl cis–trans isomerase; SUMO, small ubiquitin-like modifier; Tsf, E. coli elongation factor

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