Skip to main content
Fig. 3 | Microbial Cell Factories

Fig. 3

From: Screening for optimal protease producing Bacillus licheniformis strains with polymer-based controlled-release fed-batch microtiter plates

Fig. 3

Flowchart of the applied screening procedure for high-throughput screening in batch and fed-batch mode. Precultures were synchronized applying a two-step preculture procedure. Synchronized precultures were used to inoculate batch and fed-batch main cultures. Batch main cultures were conducted in FlowerPlates® and fed-batch main cultures in polymer-based controlled-release fed-batch microtiter plates (fed-batch MTP). The main cultures represent the screening processes in the respective mode. Cultivation conditions: FlowerPlate® (batch), fed-batch MTP (fed-batch), n = 1000 rpm, d0 = 3 mm, VL, First preculture = 0.8 mL, VL, Second preculture = 0.8 mL, VL, Main culture (batch) = 0.88 mL, VL, Main culture (fed-batch) = 0.77 mL, 30 °C

Back to article page