Fig. 1
adapted from Keil et al. [26]. ① Water diffuses into the silicone matrix and starts dissolving glucose crystals, thereby creating a highly concentrated glucose solution. ② Due to the osmotic pressure, more water diffuses into the cavity. ③ Little cracks and channels are established in the silicone matrix, which is followed by the release of the glucose solution into the culture medium
Principle of the polymer-based controlled-release fed-batch microtiter plate (fed-batch MTP). a Fed-batch MTP with cross section view into a single well. b Close-up illustration of a single round-well showing the silicone matrix with embedded glucose crystals on its bottom. c Working principle of the polymer-based controlled-release system