Fig. 5
DAP production with 10 EcLDCc-CatIB variants—comparison of linkers. Enzymatic activity of: a controls with no CatIBs, supernatant and pellet fraction of wild type EcLDCc and supernatant of EcLDCc-SG-TDoT b EcLDCc-SG variants and c EcLDCc-PT variants. Experimental conditions: Purified CatIBs from cells with an OD600 nm = 12.5, 10 mM l-lysine, 0.1 mM PLP in 50 mM Kpi buffer (pH 7.2). Sampling times: 0, 3, 6, 12 and 20 min. nA=3 (not visible, due to low standard deviation) and L6KD-Tag variants: nB=3, except 3 min SG-L6KD (nB=2). P pellet, SN supernatant. Conversion calculated based on DAP formation from l-lysine measured by HPLC