Fig. 3
Evaluation of CatIB formation by SDS-PAGE analysis. After cultivation, the optical density of the culture were normalized to OD600 nm = 12.5. The cells were disrupted and the crude cell extract was separated by centrifugation into the soluble protein containing supernatant and the insoluble CatIB‑containing pellet fractions. The pellet fraction was washed once with Milli-Q® water. The samples were diluted 1:1 with SDS sample buffer and 15 µL of each sample was loaded onto the gel and stained with SimplyBlue™ SafeStain. The molecular mass of the wildtype EcLDCc (81.88 kDa) is indicated by a red arrow. Molecular mass of negative control (empty pET28a): 0 kDa; EcLDCc-SG-TDoT: 87.66 kDa; EcLDCc-SG-18AWT: 84.22 kDa, EcLDCc-SG-L6KD: 82.85 kDa, EcLDCc-SG-GFIL8: 82.79 kDa, EcLDCc-SG-3HAMP: 100.59 kDa, EcLDCc-PT-TDoT: 88.48 kDa; EcLDCc-PT-18AWT: 85.04 kDa, EcLDCc-PT-L6KD: 83.67 kDa, EcLDCc-PT-GFIL8: 83.61 kDa, EcLDCc -PT-3HAMP: 101.41 kDa. Molecular weight determination of protein. Abbreviation: LDCWT: wild type EcLDCc control, − Negative control (empty pET28a vector)