Fig. 2
Size distribution of a EcLDCc-CatIB producer strains and b EcLDCc-CatIBs. The median (red), the 25 and 75 % Quartiles (grey box), the 5 % and 95 % whiskers and the outliers (grey cross) of the cell or CatIB area were determined for each variant. Positive control: E. coli BL21(DE3) with EcLDCc-Xa-SG-TDoT [9], negative control: E. coli BL21(DE3) with empty pET28a and wild type control: E. coli BL21(DE3) with pET28a::EcLDCc, without any linker or aggregation inducing tag. n = 100. Cpos positive control, Cneg Negative control and LDCWT wildtype EcLDCc control