Fig. 1

Microscopic images of cells producing different EcLDCc-CatIB variants and control cells at 1000-fold magnification. Cultivation was performed for 3 h at 37 °C and 69 h at 15 °C in M9-AI medium. a Positive control: E. coli BL21(DE3) with EcLDCc-Xa-SG-TDoT [9], b Negative control: E. coli BL21(DE3) with pET28a, c wild type control: E. coli BL21(DE3) with EcLDCc d EcLDCc-SG-TDoT, e EcLDCc-SG-18AWT, f EcLDCc-SG-L6KD, g EcLDCc-SG-GFIL8, h EcLDCc-SG-3HAMP, i EcLDCc-PT-TDoT, j EcLDCc-PT-18AWT, k EcLDCc-PT-L6KD, l EcLDCc-PT-GFIL8, m EcLDCc-PT-3HAMP