Fig. 6

Increases in secretion titer from optimized media and strain modifications are additive. Relative bulk secretion titer of test proteins from WT and ∆sipD strains in LB-L or LB-ES media. “LB-ES” is 10 g/L tryptone, 5 g/L yeast extract, 80 mM KH₂PO₄/K₂HPO₄ pH 7.4, 90 mM NaCl, and 0.4% w/v glycerol. Bulk relative secretion titer was normalized to ASTE13 WT in LB-L for each protein using semi-quantitative western blotting. All error bars represent standard error for three biological replicates