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Fig. 3 | Microbial Cell Factories

Fig. 3

From: An optimized growth medium for increased recombinant protein secretion titer via the type III secretion system

Fig. 3

Secretion titer increases with conductivity irrespective of species. a Relative bulk secretion titer of SptP-DH-2xFLAG-6xHis in LB supplemented with the buffers and salts listed below the graph. Species in each “level” were designed to have a similar buffer concentration (MOPS, MOPS + NaCl) and/or conductivity (NaCl, MOPS + NaCl) to the corresponding concentration of KH2PO4/K2HPO4. Each column of the table corresponds to the group of bars above it, and each row of the column corresponds to a separate bar in the group. Data was normalized to LB-L using semi-quantitative western blotting. Error bars represent standard error for four biological replicates except for black-outlined bars, which represent six biological replicates. LB-L and LB-IM are denoted by dashed lines. b Relative bulk secretion titer versus conductivity for the media in part a. The gray shading represents a 95% CI on the linear regression. Each data point is a replicate except for LB-L, which is labeled as a reference

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