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Fig. 1 | Microbial Cell Factories

Fig. 1

From: An optimized growth medium for increased recombinant protein secretion titer via the type III secretion system

Fig. 1

Medium composition affects secretion titer. a Relative bulk secretion titer of SptP-DH-2xFLAG-6xHis in LB-L, 2X YT, and TB. b Relative bulk secretion titer of SptP-DH-2xFLAG-6xHis in LB-L supplemented with the defined components in TB. Statistical significance was determined by Welch’s unequal variances t-test. Significance denoted by “***” represents p < 0.001, while “n.s.” represents p > 0.05. c Relative bulk secretion titer versus relative expression in the media from parts a, b. Expression and secretion titer were normalized to LB-L with no carbon source using densitometry measurements on western blots. All error bars represent standard error for four biological replicates

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Microbial Cell Factories

ISSN: 1475-2859

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