Table 1 Specific XR, FDH, and GDH activities in cell extracts of selected single recombinant and double recombinant clones (n = 3, avg ± SD)
Clone | Specific activity (U/mg protein) | ||
|---|---|---|---|
XRa | GDHb | FDHb | |
NcXR 1000–1 | 62.1 ± 7.1 | 0.0 | 0.21 ± 0.03 |
NcXR 4000–2 | 35.7 ± 4.0 | 0.0 | 0.29 ± 0.03 |
PsXYL1 1000–1 | 10.1 ± 0.7 | 0.0 | 0.34 ± 0.02 |
PsXYL14000-3 | 9.7 ± 1.0 | 0.0 | 0.31 ± 0.02 |
CpXR 1000–6 | 1.3 ± 0.02 | 0.0 | Not tested |
CpXR 4000–8 | 3.3 ± 0.4 | 0.0 | Not tested |
NcXR + GDH 1000–2 | 22.4 ± 0.8 | 6.7 ± 0.5 | Not tested |
NcXR + GDH 4000–1 | 38.6 ± 1.9 | 9.2 ± 0.3 | Not tested |
PsXYL1 + GDH 1000–8 | 1.7 ± 0. 2 | 4.4 ± 0.6 | Not tested |
PsXYL1 + GDH 4000–4 | 10.2 ± 2.1 | 17.4 ± 1.0 | Not tested |
CpXR + GDH 1000–5 | 0.0 | 4.6 ± 0.3 | Not tested |
CpXR + GDH 4000–6 | 0.0 | 9.4 ± 0.8 | Not tested |
pPIC4Kx empty vector control | 0.2 ± 0.1 | 0.02 ± 0.01 | Not tested |