Fig. 2

Xylitol production by (a) NcXR + GDH 4000–1 and (b) PsXYL1 + GDH 4000–4 cells. All reactions had 10 mg/mL of cells. The weight of the cells was a dry cell weight. The cells were incubated at 30 °C with 200 mM d-xylose and 0.25 mM NAD⁺ in 50 mM KPi (pH 7.0) buffer. The reactions also had 100 mM formate (red circle), 100 mM glucose (black square) or no auxiliary substrate as electron donor (blue triangle). Solid lines represent d-xylose consumption; dashed lines represent xylitol production. The data shown in the figure is the average of 3 values. All the values are within the 5% SD