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Table 2 Plasmids and oligonucleotides used/created in this study

From: Production of succinate by engineered strains of Synechocystis PCC 6803 overexpressing phosphoenolpyruvate carboxylase and a glyoxylate shunt

Primer/

Plasmid

Sequence/Construct

oligoF-AXS

TATGTCTAGAATGTGGAGTCATCCTCAGTTCGAGAAGGGTAGCGGAAGTAAAACCCGTACACAACAAAT

oligoR-ABP

TGAACTGCAGTAACTTAGATCTTTAGAACTGCGATTCTTCGG

oligoF-BBRS

CTTAAGATCTTAGTGGAGGTAAGCTTATGTGGAGTCATCCTCAGTTCGAGAAGGGTAGCGGAAGTACTGAACAGGCAACAACAACC

oligoR-BP

TGAACTGCAGTTACGCTAACAGGCGGTAGC

pEERM3 + 

Plasmid (empty backbone) for integration into neutral site of Synechocystis 6803, based on the pJ344 vector backbone, carrying chloramphenicol resistance cassette, PnrsB promoter and the synthetic RBS, RBS*

pEERM3-aceA

As pEERM3, but with E. coli aceA expressed from the PnrsB promoter

pEERM3-aceAB

As pEERM3, but with E. coli aceAB expressed from the PnrsB promoter