Fig. 2

SDS-PAGE and Western immunoblot on extracts of the Synechocystis strains created in this study. SDS-PAGE/Western immunoblot analysed for the presence of ICL and MS (a); analysed for the presence of PEPc (b). a Upper panel shows SDS-PAGE loaded with 5 µg of protein crude extract from the Synechocystis engineered strains created in this study (Table 1). Lower panel shows Western immunoblot using anti-Strep antibody for the Synechocystis engineered strains (Table1). Bands correspond to MS (approximate molecular weight 60 kDa) and to ICL (approximate molecular weight 47 kDa). Samples are from cells incubated under darkness. b Upper panel shows SDS-PAGE loaded with 31 µg of protein crude extract from Synechocystis strains created in this study (Table 1). Lower panel displays Western immunoblot using anti-PEPc for the Synechocystis engineered strains. The approximate molecular weight of PEPc is 110 kDa. Control (CTL) corresponds to purified PEPc from the cyanobacterium Synechocystis PCC 6803. Samples are from cells incubated under anoxic darkness