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Table 1 Strains and plasmids

From: Fine tuning the glycolytic flux ratio of EP-bifido pathway for mevalonate production by enhancing glucose-6-phosphate dehydrogenase (Zwf) and CRISPRi suppressing 6-phosphofructose kinase (PfkA) in Escherichia coli

Strain and plasmids

Relevant properties

Sources

Strains

 E. coli BW25113

F- Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514

Lab stock

 BW-P

BW25113 derivative, ΔpfkA::FRT

[13]

 BW-P 23100-zwf

BW-P derived, zwf promoter:: BBa-J 23100, CmR

This study

 BW-P 23104-zwf

BW-P derived, zwf promoter:: BBa-J 23104, CmR

This study

 BW-P 23105-zwf

BW-P derived, zwf promoter:: BBa-J 23105, CmR

This study

 BW-P 23108-zwf

BW-P derived, zwf promoter:: BBa-J 23108, CmR

This study

 BW-P 23114-zwf

BW-P derived, zwf promoter:: BBa-J 23114, CmR

This study

 BW-P10 BF

BW-P 23100-zwf carrying pBSA pFF

This study

 BW-P04 BF

BW-P 23104-zwf carrying pBSA pFF

This study

 BW-P 05 BF

BW-P 23105-zwf carrying pBSA pFF

This study

 BW-P 08 BF

BW-P 23108-zwf carrying pBSA pFF

This study

 BW-P 14 BF

BW-P 23114-zwf carrying pBSA pFF

This study

 BPB10F

BW-P 23100-zwf carrying pBSA-23100 pBSA

This study

 BPB02F

BW-P 23100-zwf carrying pBSA-23102 pBSA

This study

 BPB04F

BW-P 23100-zwf carrying pBSA-23104 pBSA

This study

 BPB18F

BW-P 23100-zwf carrying pBSA-23118 pBSA

This study

 BPB19F

BW-P 23100-zwf carrying pBSA-23119 pBSA

This study

 BW25113 23100-zwf

BW25113 derived, zwf promoter:: BBa-J 23100

This study

 BiBF1

BW25113 23100-zwf carrying pBSA-dCas9 pFF-sgRNA1

This study

 BiBF2

BW25113 23100-zwf carrying pBSA-dCas9 pFF-sgRNA2

This study

 BiBF3

BW25113 23100-zwf carrying pBSA-dCas9 pFF-sgRNA3

This study

 BiB1F

BW25113 23100-zwf carrying pBSA-sgRNA1 pFF- dCas9

This study

 BiB2F

BW25113 23100-zwf carrying pBSA-sgRNA2 pFF- dCas9

This study

 BiB3F

BW25113 23100-zwf carrying pBSA-sgRNA3 pFF- dCas9

This study

 BB1F

BW25113 23100-zwf carrying pBSA-sgRNA1 pFF

This study

 BBFd

BW25113 23100-zwf carrying pBSA pFF- dCas9

This study

 BiBd1F

BW25113 23100-zwf carrying pBSA-sgRNA1-dCas9 pFF

This study

 pFF

pCDFduet, with fxpk from B. Adolescentis, and fbp gene from E. coli, AmpR

[13]

 pBSA

pTrc99a with atoB from E. coli, mvaS and mvaA from L. casei. tac promoter, AmpR

[13]

 pTKRED

ParaBAD promoter containing plasmid, SpeR

[34]

 pCP20

Helper plasmid expressing FLP recombinase, ts-rep, AmpR, CmR

[35]

 pKD3

Template plasmid with CmR gene and FLP recognition target

[35]

 P23100-GFP

pTrc99a with BBa-J 23100 promoter and green fluorescent protein sequence, AmpR

This study

 P23104-GFP

pTrc99a with BBa-J 23104 promoter and green fluorescent protein sequence, AmpR

This study

 P23105-GFP

pTrc99a with BBa-J 23105 promoter and green fluorescent protein sequence, AmpR

This study

 P23108-GFP

pTrc99a with BBa-J 23108 promoter and green fluorescent protein sequence, AmpR

This study

 P23114-GFP

pTrc99a with BBa-J 23114 promoter and green fluorescent protein sequence, AmpR

This study

 Pzwf-GFP

pTrc99a with zwf promoter from E. coli BW25113 and green fluorescent protein sequence, AmpR

This study

 pBSA-23119

pBSA with BBa-J 23119 promoter

This study

 PBSA-23100

pBSA with BBa-J 23100 promoter

This study

 PBSA-23102

pBSA with BBa-J 23102 promoter

This study

 PBSA-23104

pBSA with BBa-J 23104 promoter

This study

 PBSA-23118

pBSA with BBa-J 23118 promoter

This study

 pFF-sgRNA1

pFF containing pfkA sgRNA1, SpcR

This study

 pFF-sgRNA2

pFF containing pfkA sgRNA2, SpcR

This study

 pFF-sgRNA3

pFF containing pfkA sgRNA3, SpcR

This study

 pBSA-dCas9

pBSA containing Cas9 array, AmpR

This study

 pBSA-sgRNA1

pBSA containing pfkA sgRNA1, AmpR

This study

 pBSA-sgRNA2

pBSA containing pfkA sgRNA2, AmpR

This study

 pBSA-sgRNA3

pBSA containing pfkA sgRNA3, AmpR

This study

 pFF-dCas9

pFF containing Cas9 array, AmpR

This study

 pFF-sgRNA1-dCas9

pFF containing Cas9 array and pfkA sgRNA1, AmpR

This study

 pBSA-23102-sgRNA1

pBSA-23102 containing pfkA sgRNA1, AmpR

This study