Strain and plasmids | Relevant properties | Sources |
---|---|---|
Strains | ||
E. coli BW25113 | F- Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514 | Lab stock |
BW-P | BW25113 derivative, ΔpfkA::FRT | [13] |
BW-P 23100-zwf | BW-P derived, zwf promoter:: BBa-J 23100, CmR | This study |
BW-P 23104-zwf | BW-P derived, zwf promoter:: BBa-J 23104, CmR | This study |
BW-P 23105-zwf | BW-P derived, zwf promoter:: BBa-J 23105, CmR | This study |
BW-P 23108-zwf | BW-P derived, zwf promoter:: BBa-J 23108, CmR | This study |
BW-P 23114-zwf | BW-P derived, zwf promoter:: BBa-J 23114, CmR | This study |
BW-P10 BF | BW-P 23100-zwf carrying pBSA pFF | This study |
BW-P04 BF | BW-P 23104-zwf carrying pBSA pFF | This study |
BW-P 05 BF | BW-P 23105-zwf carrying pBSA pFF | This study |
BW-P 08 BF | BW-P 23108-zwf carrying pBSA pFF | This study |
BW-P 14 BF | BW-P 23114-zwf carrying pBSA pFF | This study |
BPB10F | BW-P 23100-zwf carrying pBSA-23100 pBSA | This study |
BPB02F | BW-P 23100-zwf carrying pBSA-23102 pBSA | This study |
BPB04F | BW-P 23100-zwf carrying pBSA-23104 pBSA | This study |
BPB18F | BW-P 23100-zwf carrying pBSA-23118 pBSA | This study |
BPB19F | BW-P 23100-zwf carrying pBSA-23119 pBSA | This study |
BW25113 23100-zwf | BW25113 derived, zwf promoter:: BBa-J 23100 | This study |
BiBF1 | BW25113 23100-zwf carrying pBSA-dCas9 pFF-sgRNA1 | This study |
BiBF2 | BW25113 23100-zwf carrying pBSA-dCas9 pFF-sgRNA2 | This study |
BiBF3 | BW25113 23100-zwf carrying pBSA-dCas9 pFF-sgRNA3 | This study |
BiB1F | BW25113 23100-zwf carrying pBSA-sgRNA1 pFF- dCas9 | This study |
BiB2F | BW25113 23100-zwf carrying pBSA-sgRNA2 pFF- dCas9 | This study |
BiB3F | BW25113 23100-zwf carrying pBSA-sgRNA3 pFF- dCas9 | This study |
BB1F | BW25113 23100-zwf carrying pBSA-sgRNA1 pFF | This study |
BBFd | BW25113 23100-zwf carrying pBSA pFF- dCas9 | This study |
BiBd1F | BW25113 23100-zwf carrying pBSA-sgRNA1-dCas9 pFF | This study |
pFF | pCDFduet, with fxpk from B. Adolescentis, and fbp gene from E. coli, AmpR | [13] |
pBSA | pTrc99a with atoB from E. coli, mvaS and mvaA from L. casei. tac promoter, AmpR | [13] |
pTKRED | ParaBAD promoter containing plasmid, SpeR | [34] |
pCP20 | Helper plasmid expressing FLP recombinase, ts-rep, AmpR, CmR | [35] |
pKD3 | Template plasmid with CmR gene and FLP recognition target | [35] |
P23100-GFP | pTrc99a with BBa-J 23100 promoter and green fluorescent protein sequence, AmpR | This study |
P23104-GFP | pTrc99a with BBa-J 23104 promoter and green fluorescent protein sequence, AmpR | This study |
P23105-GFP | pTrc99a with BBa-J 23105 promoter and green fluorescent protein sequence, AmpR | This study |
P23108-GFP | pTrc99a with BBa-J 23108 promoter and green fluorescent protein sequence, AmpR | This study |
P23114-GFP | pTrc99a with BBa-J 23114 promoter and green fluorescent protein sequence, AmpR | This study |
Pzwf-GFP | pTrc99a with zwf promoter from E. coli BW25113 and green fluorescent protein sequence, AmpR | This study |
pBSA-23119 | pBSA with BBa-J 23119 promoter | This study |
PBSA-23100 | pBSA with BBa-J 23100 promoter | This study |
PBSA-23102 | pBSA with BBa-J 23102 promoter | This study |
PBSA-23104 | pBSA with BBa-J 23104 promoter | This study |
PBSA-23118 | pBSA with BBa-J 23118 promoter | This study |
pFF-sgRNA1 | pFF containing pfkA sgRNA1, SpcR | This study |
pFF-sgRNA2 | pFF containing pfkA sgRNA2, SpcR | This study |
pFF-sgRNA3 | pFF containing pfkA sgRNA3, SpcR | This study |
pBSA-dCas9 | pBSA containing Cas9 array, AmpR | This study |
pBSA-sgRNA1 | pBSA containing pfkA sgRNA1, AmpR | This study |
pBSA-sgRNA2 | pBSA containing pfkA sgRNA2, AmpR | This study |
pBSA-sgRNA3 | pBSA containing pfkA sgRNA3, AmpR | This study |
pFF-dCas9 | pFF containing Cas9 array, AmpR | This study |
pFF-sgRNA1-dCas9 | pFF containing Cas9 array and pfkA sgRNA1, AmpR | This study |
pBSA-23102-sgRNA1 | pBSA-23102 containing pfkA sgRNA1, AmpR | This study |