Fig. 5
From: Synthesis of cembratriene-ol and cembratriene-diol in yeast via the MVA pathway
Production of CBT-diols in yeast strain BY-T20 expressing AD-CBTS1 and BD-CYP450. a GC–MS spectrums of yeast extract. The GC–MS peak for CBT-diol is indicated. b The associated mass peaks of the yeast produced CBT-diol. Bold numbers indicate the specific mass peaks discriminating CBT-diol from CBT-ol. c Yeast growth and CBT-diol production by bioreactor cultivation in the optimized medium. WW wet weight. d UPLC detection of CBT-diols in the extract of yeast culture. Peaks for α-CBT-diol and β-CBT-diol are indicated. e Content of CBT-diols in the indicated yeast culture or cultivation broth. Each values is the average of triplicates. N.D. indicates not detected, and bars indicate mean + SD