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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Comparative genomics and transcriptomics analyses provide insights into the high yield and regulatory mechanism of Norvancomycin biosynthesis in Amycolatopsis orientalis NCPC 2-48

Fig. 4

AoStrR1 and AoLuxR1 are cluster-specific positive regulators for norvancomycin biosynthesis. a The expression level of four potential regulatory genes at each time points on RNA-seq assay. The gene expression quantification is based on the FPKM (fragments per kilobase of exon per million fragments mapped) value. b, d Norvancomycin yield in the fermentation product of the original strain CPCC 200066 and AoStrR1/AoLuxR1-overexpressed strains. p2A and p2A-ermE*p represent two overexpressed strains which contain its native promoter or ermE*p strong promoter. Values are presented as means + SEM (two biological repeats for each strain). c, e Transcriptional analysis of nvcm genes in CPCC 200066 and AoStrR1/AoLuxR1-overexpressed strains. The mycelia of each strain were collected at 36 h after fermentation for the extraction of total RNAs, and then these samples were subjected to RT-qPCR analysis. The CT values of the target genes were normalized to the principal sigma factor gene hrdB. The relative expression level of each sample was represented as the value related to the sample CPCC 200066. Values are presented as means ± SEM (three biological repeats for each strain)

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Microbial Cell Factories

ISSN: 1475-2859

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