Table 2 The primers used in this study
Primer | Characteristic | Source |
|---|---|---|
Vio-pETduet-PF | gcgatcgctgacgtcggtaccATGAAGCATTCTTCCGATATCTGC | This study |
Vio-pETduet-PR | tttaccagactcgagggtaccCTAGCGCTTGGCGGCGAAG | This study |
dVio-PR | CGGTTCCCGGTTTTCCATCAGTTGACCCTCCCTATC | This study |
dVio-PF | GATAGGGAGGGTCAACTGATGGAAAACCGGGAACCG | This study |
vioB-RBSm-PF1 | CATGACCGTTCGGGGAGCACATGAGC | This study |
vioB-RBSm-PR1 | AATGCTCATGTGCTCCCCGAACGGTC | This study |
vioB -RBSm-PF2 | CATGACCGTTCAAGAAACACATGAGC | This study |
vioB-RBSm-PR2 | AATGCTCATGTGTTTCTTGAACGGTC | This study |
vioB-RBSm-PF3 | CATGACCGTTCAAGGAGCACATGAGC | This study |
vioB-RBSm-PR3 | AATGCTCATGTGCTCCTTGAACGGTC | This study |
vioC-RBSm-PF | TCTAGAGAGGCCTGATGAAAAGAGCAATC | This study |
vioC-RBSm-PR | GATTGCTCTTTTCATCAGGCCTCTCTAGA | This study |
vioD-RBSm-PF1 | TACAAGATAGGGAGGAACTGATGAAGATTC | This study |
vioD-RBSm-PR1 | GAATCTTCATCAGTTCCTCCCTATCTTGTA | This study |
vioD-RBSm-PF2 | TACAAGATAGGGAGGTGATGAAGATTCTGG | This study |
vioD-RBSm-PR2 | CCAGAATCTTCATCACCTCCCTATCTTGTA | This study |
vioE-RBSm-PF | GCTGCAACGCTGAAGGAGCCGCATGGAAAAC | This study |
vioE-RBSm-PR | GTTTTCCATGCGGCTCCTTCAGCGTTGCAGC | This study |
tnaA-KO-us arm-PF | TTGAGTATTATACTGTAG | This study |
tnaA-KO-us arm-PR | GGTCGACGGATCCCCGGAATTGCCACCATTTTGCTGCG | This study |
tnaA-KO-PF | GGATCGCAGCAAAATGGTGGCAATTCCGGGGATCCGTCGACC | This study |
tnaA-KO-PR | CGCCAATCTCTTCCAGACCATCTGTAGGCTGGAGCTGCTTC | This study |
tnaA -KO-ds arm-PF | GAAGCAGCTCCAGCCTACAGATGGTCTGGAAGAGATTG | This study |
tnaA-KO-ds arm-PR | GGTGTTACCGATTAAATC | This study |
tnaA-KOV-PF | TTGAGTATTATACTGTAG | This study |
tnaA-KOV-PR | GCGTGATAGCCCAAATTC | This study |