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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Towards a 'chassis' for bacterial magnetosome biosynthesis: genome streamlining of Magnetospirillum gryphiswaldense by multiple deletions

Fig. 3

Overview over identified active mobile genetic elements in M. gryphiswaldense. a One group of active insertion elements (ISMgr2tnpA and ISMgr2tnpB, green) belongs to the IS3 family, all three copies were deleted in this study. b The second variant of this group contains two hypothetical genes (black) and an ISMgr2tnpB gene. Both copies were deleted. c A further group of active mobile elements is represented by a transposon tandem (tn-tandem). The first transposon (tn1) of the tn-tandem is a member of the putative transposase of IS4/5 family and contains a DUF4096 domain known to bind the end of a transposon and to catalyze the movement of the transposon to another part of the genome by cut and paste or replicative transposition mechanism. The second tn-tandem transposon (tn2) contains a DDE domain, named after a conserved amino acid triad Asp, Asp, Glu, the active site [57]. d tn2 is present in two single copies in the WT genome. Two copies of tn-tandem were deleted in ∆TZ-16::MAG-gusA and ∆TZ-17::MAG-gusA

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