Skip to main content
Fig. 7 | Microbial Cell Factories

Fig. 7

From: Investigation of Kluyveromyces marxianus as a novel host for large‐scale production of porcine parvovirus virus‐like particles

Fig. 7

SDS-PAGE (a) and HPLC (b) assays of the purified PPV VLPs remained in the pH adjusted precipitates. The precipitates were dissolved using 20 mM Tris-HCl buffer pH 8.0. After clarification by centrifugation, supernatants were loaded onto a Capto Q XP column and PPV VLPs were eluted with 20 mM Tris-HCl pH 7.4 containing 0.5 M NaCl. Elution fractions were ultrafiltrated with a 750 kDa column in a tangential flow filtration. Lane RS: the redissolved supernatant; Lane F: flowthrough fractions; Lane E: elution fractions; Lane U; the ultrafiltrated VLPs

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us