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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Investigation of Kluyveromyces marxianus as a novel host for large‐scale production of porcine parvovirus virus‐like particles

Fig. 6

Scale-up purification of PPV VLPs. a SDS-PAGE analysis of the purified PPV VLPs samples from IEX and Gel filtration. Lane M, PageRuler Prestained Protein Ladder; Lane 1, cell lysates prepared by PBS pH 7.4; lane 2, the supernatant of cell lysates adjusted to pH 4.0; Lane 3, elution fraction of Capto S ImpAct exchange chromatography; Lane 4, elution of Sephacryl® S-500 Gel filtration. b TEM scan of the Gel filtration purified PPV VLPs. Bar, 100 nm. c HPLC analysises of the supernatant pH adjusted cell lysate (c), the IEX purified PPV VLPs (d), and the gel filtration purified PPV VLPs (e)

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Microbial Cell Factories

ISSN: 1475-2859

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